Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8 ng/ml determined by ELISA. (QC Test)
Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay (Gator® Prime).

CD40 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 46.2 kDa and the accession number is A0A0S2Z3C7.

Human

HEK293 Cells

TNFRSF5,p50,CDW40,CD40 molecule, TNF receptor superfamily member 5,Bp50

Glu21-Arg193

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy