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Anti-CD44 Antibody (2B300)
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Catalog No. TMAH-00199
Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA,IHC,WB.
Anti-CD44 Antibody (2B300)
Anti-CD44 Antibody (2B300)
😃Good
Catalog No. TMAH-00199Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Chemical Properties
| Description | Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA,IHC,WB. |
| Synonyms | Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group) |
| Ig Type | Rabbit IgG |
| Clone | 2B300 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysate -All lanes CD44 antibody at 1.25μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 82 KDa -Observed band size: 82 KDa 2. IHC image of TMAH-00199 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus. |
| Tissue Specificity | Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide |
| Antigen Species | Human |
| Gene Name | CD44 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Immunology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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