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Anti-CD44 Antibody (2B300)

😃Good
Catalog No. TMAH-00199
Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)

Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA, WB, IHC.

Anti-CD44 Antibody (2B300)

Anti-CD44 Antibody (2B300)

😃Good
Catalog No. TMAH-00199Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD44 Antibody (2B300) is an antibody targeting CD44. Anti-CD44 Antibody (2B300) can be used in ELISA, WB, IHC.
AliasPgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Ig Type
Rabbit IgG
Clone
2B300
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysate
-All lanes CD44 antibody at 1.25μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 82 KDa
-Observed band size: 82 KDa
2. IHC image of TMAH-00199 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:500.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Cell projection, microvillus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide
Antigen Species
Human
Gene ID
960
Uniprot ID
Biology Area
Immunology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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