Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IP

Reactivity: Human, Mouse, Rat

Rabbit IgG

2V859

Human, Mouse, Rat

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue
-All lanes: DHFR antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 22, 16 kDa
-Observed band size: 22 kDa
2. IHC image of TMAH-00344 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00344 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
4. Immunoprecipitating DHFR in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00344 in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00344(2µg)+ Hela whole cell lysate(500µg)
-Lane 3: Hela whole cell lysate (10µg)

ELISA, WB, IHC, IP

WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human DHFR

Human

1719

Cancer, Cell biology, Metabolism, Signal transduction