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Anti-METTL1 Antibody (3N140)

😃Good
Catalog No. TMAY-01888
Alias YDL201w, TRMT8, TRM8, methyltransferase like 1, C12orf1

Anti-METTL1 Antibody (3N140) is a Mouse antibody targeting METTL1. Anti-METTL1 Antibody (3N140) can be used in WB,ELISA,ELISA(Det),IP.

Anti-METTL1 Antibody (3N140)

Anti-METTL1 Antibody (3N140)

😃Good
Catalog No. TMAY-01888Alias YDL201w, TRMT8, TRM8, methyltransferase like 1, C12orf1
Anti-METTL1 Antibody (3N140) is a Mouse antibody targeting METTL1. Anti-METTL1 Antibody (3N140) can be used in WB,ELISA,ELISA(Det),IP.
Pack SizePriceAvailabilityQuantity
20 μL$69 7-10 days
100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-METTL1 Antibody (3N140) is a Mouse antibody targeting METTL1. Anti-METTL1 Antibody (3N140) can be used in WB,ELISA,ELISA(Det),IP.
AliasYDL201w, TRMT8, TRM8, methyltransferase like 1, C12orf1
Ig Type
Monoclonal Mouse IgG1
Clone
3N140
Reactivity
Human
Verified Activity
1. Anti-METTL1 mouse monoclonal antibody at 1:500 dilution.

-Lane A: 293T Whole Cell Lysate.

-Lane B: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:31 kDa.

-Observed band size:31 kDa.

2. METTL1 was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-Lane B:0.5 mg Jurkat Whole Cell Lysate.

-2 µL anti-METTL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-METTL1 mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 34 kDa.

-Observed band size: 34 kDa
Application
WB,ELISA,ELISA(Det),IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (rh METTL1; TMPY-01661; NP_005362.3; Asp 32-Gln 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
Research BackgroundtRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human METTL1 protein (TMPY-01661)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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