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Anti-MAP2K1 Antibody (6F541)
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Catalog No. TMAH-00724
Alias PRKMK1, MKK1, MEK 1, MAPKK 1, MAPK/ERK kinase 1, MAP kinase kinase 1, ERK activator kinase 1, EC 2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1
Anti-MAP2K1 Antibody (6F541) is an antibody targeting MAP2K1. Anti-MAP2K1 Antibody (6F541) can be used in ELISA, WB, IHC, IP.
Anti-MAP2K1 Antibody (6F541)
Anti-MAP2K1 Antibody (6F541)
😃Good
Catalog No. TMAH-00724Alias PRKMK1, MKK1, MEK 1, MAPKK 1, MAPK/ERK kinase 1, MAP kinase kinase 1, ERK activator kinase 1, EC 2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1
Anti-MAP2K1 Antibody (6F541) is an antibody targeting MAP2K1. Anti-MAP2K1 Antibody (6F541) can be used in ELISA, WB, IHC, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IP Reactivity: Human |
| Alias | PRKMK1, MKK1, MEK 1, MAPKK 1, MAPK/ERK kinase 1, MAP kinase kinase 1, ERK activator kinase 1, EC 2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1 |
| Ig Type | Rabbit IgG |
| Clone | 6F541 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate -All lanes: MAP2K1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 44, 41 kDa -Observed band size: 44 kDa 2. IHC image of TMAH-00724 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunoprecipitating MAP2K1 in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00724 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00724(2µg)+ Hela whole cell lysate(500µg) -Lane 3: Hela whole cell lysate (10µg) |
| Application | ELISA, WB, IHC, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Membrane; Peripheral membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human MEK1 |
| Antigen Species | Human |
| Gene ID | 5604 |
| Uniprot ID | |
| Biology Area | Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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