Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, IF, FCM

Reactivity: Human

Mouse IgG2a

6Z293

Human

1. Immunofluorescence staining of HEPG2 cell with TMAH-00062 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
2. Immunofluorescence staining of A549 cell with TMAH-00062 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
3. Overlay Peak curve showing 293 cells surface stained with TMAH-00062 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.

ELISA, IF, FCM

IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human ANPEP Protein

Human

290

Immunology, Stem cells