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CTLA-4 Protein, Human, Recombinant

(Synonyms: IDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5) Copy Product Info

Synonyms: IDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5

Catalog No. TMPY-04824 Copy Product Info
CTLA-4 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P16410-1.

Batch Information

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Purity:SDS-PAGE: 97.5%; SEC-HPLC: 99.2%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized CTLA-4 Protein, Human, Recombinant at 2 μg/mL (100 μL/well) can bind B7-1 Protein, Human, Recombinant (His & hFc Tag), Biotinylated, the EC50 of B7-1 is 6-27 ng/mL.
CTLA-4 Protein, Human, Recombinant
Description
CTLA-4 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P16410-1.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberP16410-1
ConstructionA DNA sequence encoding the human CTLA4 (NP_005205.2) (Met1-Asp161) was expressed. Predicted N terminal: Lys 36
Protein Purity
> 95 % as determined by SDS-PAGE.
CTLA-4 Protein, Human, Recombinant
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsIDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5
Research Background
Chemical Properties
Molecular Weight13.5 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: CTLA-4 Protein, Human, Recombinant chemical structure | CTLA-4 Protein, Human, Recombinant molecular weight