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Anti-Wilms tumor/WT1 Antibody (4A565)
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Catalog No. TMAH-01253
Anti-Wilms tumor/WT1 Antibody (4A565) is an antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (4A565) can be used in ELISA, WB, IHC.
Anti-Wilms tumor/WT1 Antibody (4A565)
Anti-Wilms tumor/WT1 Antibody (4A565)
😃Good
Catalog No. TMAH-01253
Anti-Wilms tumor/WT1 Antibody (4A565) is an antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (4A565) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Ig Type | Rabbit IgG |
| Clone | 4A565 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate -All lanes: Wilms Tumor Protein antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 50, 48, 48, 49, 35, 57, 56, 34 kDa -Observed band size: 50 kDa 2. IHC image of TMAH-01253 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Nucleus, nucleolus. Cytoplasm.; [Isoform 1]: Nucleus speckle.; [Isoform 4]: Nucleus, nucleoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Wilms Tumor Protein |
| Antigen Species | Human |
| Gene ID | 7490 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Tags & Cell Markers |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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