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Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
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Catalog No. TMAY-02237
Alias purine nucleoside phosphorylase, PUNP, PRO1837, NP
Anti-Nucleoside phosphorylase/PNP Antibody (1O563) is a Rabbit antibody targeting Nucleoside phosphorylase/PNP. Anti-Nucleoside phosphorylase/PNP Antibody (1O563) can be used in WB,ELISA,IP.
Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
😃Good
Catalog No. TMAY-02237Alias purine nucleoside phosphorylase, PUNP, PRO1837, NP
Anti-Nucleoside phosphorylase/PNP Antibody (1O563) is a Rabbit antibody targeting Nucleoside phosphorylase/PNP. Anti-Nucleoside phosphorylase/PNP Antibody (1O563) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Alias | purine nucleoside phosphorylase, PUNP, PRO1837, NP |
| Ig Type | Rabbit IgG |
| Clone | 1O563 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PNP3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:32 kDa. -Observed band size:32 kDa. 2. PNP-3 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-PNP-3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PNP-3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 33 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine nucleoside phosphorylase / PNP (rh Purine nucleoside phosphorylase / PNP; TMPY-02279; P00491; Met 1-Ser 289). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Purine nucleoside phosphorylase / PNP protein (TMPY-02279) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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