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Anti-VCP Antibody (6Z342)
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Catalog No. TMAH-01242
Alias VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit
Anti-VCP Antibody (6Z342) is an antibody targeting VCP. Anti-VCP Antibody (6Z342) can be used in ELISA, WB, IHC, IF, IP.
Anti-VCP Antibody (6Z342)
Anti-VCP Antibody (6Z342)
😃Good
Catalog No. TMAH-01242Alias VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit
Anti-VCP Antibody (6Z342) is an antibody targeting VCP. Anti-VCP Antibody (6Z342) can be used in ELISA, WB, IHC, IF, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IF, IP Reactivity: Human, Rat |
| Alias | VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit |
| Ig Type | Rabbit IgG |
| Clone | 6Z342 |
| Reactivity | Human, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue -All lanes: VCP antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 90 kDa -Observed band size: 90 kDa 2. IHC image of TMAH-01242 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-01242 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunofluorescence staining of SY5Y Cells with TMAH-01242 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 5. Immunoprecipitating VCP in U251 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-01242 in U251 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-01242(2µg)+ U251 whole cell lysate(500µg) -Lane 3: U251 whole cell lysate (10µg) |
| Application | ELISA, WB, IHC, IF, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Cytoplasm, Stress granule. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Together with SPRTN metalloprotease, involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis. Involved in interstrand cross-link repair in response to replication stress by mediating unloading of the ubiquitinated CMG helicase complex. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human VCP |
| Antigen Species | Human |
| Gene ID | 7415 |
| Uniprot ID | |
| Biology Area | Neuroscience, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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