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Anti-Galectin-3/LGALS3 Antibody (5L119)
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Catalog No. TMAY-00557
Anti-Galectin-3/LGALS3 Antibody (5L119) is a Mouse antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Antibody (5L119) can be used in WB,ELISA,IP.
Anti-Galectin-3/LGALS3 Antibody (5L119)
Anti-Galectin-3/LGALS3 Antibody (5L119)
😃Good
Catalog No. TMAY-00557
Anti-Galectin-3/LGALS3 Antibody (5L119) is a Mouse antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Antibody (5L119) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Ig Type | Mouse IgG1 |
| Clone | 5L119 |
| Reactivity | Human |
| Verified Activity | 1. Galectin3 was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -4 µL anti-Galectin3 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Galectin3 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 30 kDa. -Observed band size: 30 kDa. 2. Anti-Galectin3 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: SW480 Whole Cell Lysate. -Lane C: A431 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:31 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Galectin-3 / LGALS3 (rh Galectin-3 / LGALS3; P17931Met1-Ile250). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Galectin-3 / LGALS3 protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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