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Anti-PSGL-1/CD162 Antibody (4P646)
🥰Excellent
Catalog No. TMAY-02648
Anti-PSGL-1/CD162 Antibody (4P646) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (4P646) can be used in WB,ELISA,IP.
Anti-PSGL-1/CD162 Antibody (4P646)
Anti-PSGL-1/CD162 Antibody (4P646)
🥰Excellent
Catalog No. TMAY-02648
Anti-PSGL-1/CD162 Antibody (4P646) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (4P646) can be used in WB,ELISA,IP.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PSGL-1/CD162 Antibody (4P646) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (4P646) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4P646 |
| Reactivity | Human |
| Verified Activity | 1. Anti-SELPLG mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:120 kDa. 2. SELPLG was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -1 µL anti-SELPLG mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SELPLG mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 43 kDa. -Observed band size: 120 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSGL-1 / CD162 / SELPLG (rh PSGL-1 / CD162 / SELPLG; TMPY-03531; Q14242-1; Met1-Gly295). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human PSGL-1 / CD162 / SELPLG Protein (TMPY-03531) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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