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Anti-PAK2 Antibody (5W642)
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Catalog No. TMAH-00851
Alias Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1
Anti-PAK2 Antibody (5W642) is an antibody targeting PAK2. Anti-PAK2 Antibody (5W642) can be used in ELISA, WB, FCM, IP.
Anti-PAK2 Antibody (5W642)
Anti-PAK2 Antibody (5W642)
😃Good
Catalog No. TMAH-00851Alias Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1
Anti-PAK2 Antibody (5W642) is an antibody targeting PAK2. Anti-PAK2 Antibody (5W642) can be used in ELISA, WB, FCM, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM, IP Reactivity: Human, Mouse, Rat |
| Alias | Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1 |
| Ig Type | Rabbit IgG |
| Clone | 5W642 |
| Reactivity | Human, Mouse, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue -All lanes: PAK2 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 59 kDa -Observed band size: 59 kDa 2. Overlay histogram showing HepG2 cells stained with TMAH-00851 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. 3. Immunoprecipitating PAK2 in Raji whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00851 in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00851(2µg)+ Raji whole cell lysate(500µg) -Lane 3: Raji whole cell lysate (10µg) |
| Application | ELISA, WB, FCM, IP |
| Recommended Dose | WB:1:500-1:5000; FCM:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | [Serine/threonine-protein kinase PAK 2]: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human PAK2 |
| Antigen Species | Human |
| Gene ID | 5062 |
| Uniprot ID | |
| Biology Area | Neuroscience, Cancer, Cell biology, Microbiology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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