TSLP Protein, Human, Recombinant (His)

(Synonyms: TSLP, thymic stromal lymphopoietin) Copy Product Info

Synonyms: TSLP, thymic stromal lymphopoietin

Catalog No. TMPK-00037 Copy Product Info

TSLP Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 16.54 kDa and the accession number is Q969D9-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$58	7-10 days	7-10 days
10 µg	$92	7-10 days	7-10 days
20 µg	$148	7-10 days	7-10 days
50 µg	$289	7-10 days	7-10 days
100 µg	$487	7-10 days	7-10 days
200 µg	$883	7-10 days	7-10 days
500 µg	$1,950	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human TSLP, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 15.8 ng/ml determined by ELISA. (QC Test) Immobilized Human TSLP, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA. Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP, His Tag with an affinity constant of 31.30 nM as determined in BLI assay .
Description	TSLP Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 16.54 kDa and the accession number is Q969D9-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q969D9-1
Construction	Tyr29-Gln159
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	<0.1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	TSLP, thymic stromal lymphopoietin
Research Background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Chemical Properties

Molecular Weight

16.54 kDa (predicted), 12 kDa and 20-25 kDa (due to furin cleavage site)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.