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Anti-Cystatin S Antibody (1O63)
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Catalog No. TMAY-01897
Alias MGC71923, cystatin S, CST4
Anti-Cystatin S Antibody (1O63) is a Rabbit antibody targeting Cystatin S. Anti-Cystatin S Antibody (1O63) can be used in ELISA,FCM,ICC/IF.
Anti-Cystatin S Antibody (1O63)
Anti-Cystatin S Antibody (1O63)
😃Good
Catalog No. TMAY-01897Alias MGC71923, cystatin S, CST4
Anti-Cystatin S Antibody (1O63) is a Rabbit antibody targeting Cystatin S. Anti-Cystatin S Antibody (1O63) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Cystatin S Antibody (1O63) is a Rabbit antibody targeting Cystatin S. Anti-Cystatin S Antibody (1O63) can be used in ELISA,FCM,ICC/IF. |
| Alias | MGC71923, cystatin S, CST4 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1O63 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CST4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CST4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human CST4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CST4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin S / CST4 (rh Cystatin S / CST4; TMPY-01644; NP_001890.1; Met 1-Ala 141). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to thecystatin family. Cystatin-4 / CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4 / CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4 / CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Cystatin S / CST4 protein (TMPY-01644) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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