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Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
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Catalog No. TMAH-00951
Alias Phospho-PTPN11 (Y542)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) is an antibody targeting Phospho-PTPN11 (Tyr542). Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) can be used in ELISA, WB, IP.
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687)
😃Good
Catalog No. TMAH-00951Alias Phospho-PTPN11 (Y542)
Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) is an antibody targeting Phospho-PTPN11 (Tyr542). Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) can be used in ELISA, WB, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) is an antibody targeting Phospho-PTPN11 (Tyr542). Anti-Phospho-PTPN11 (Tyr542) Antibody (3Q687) can be used in ELISA, WB, IP. |
| Alias | Phospho-PTPN11 (Y542) |
| Ig Type | Rabbit IgG |
| Clone | 3Q687 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate(treated with Pervanadate or not) -All lanes Phospho-PTPN11 antibody at 0.65μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 68 KDa -Observed band size: 68 KDa 2. Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate -Lane 1: Rabbit control IgG(1μg)instead of TMAH-00951 in Hela whole cell lysate treated with Pervanadate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00951(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg) -Lane 3: Hela whole cell lysate treated with Pervanadate(20μg) |
| Application | ELISA, WB, IP |
| Recommended Dose | WB:1:500-1:5000; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-PTPN11 (Y542) |
| Antigen Species | Human |
| Gene ID | 5781 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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