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Anti-EIF5A Antibody (4P149)
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Catalog No. TMAH-00383
Alias eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A
Anti-EIF5A Antibody (4P149) is an antibody targeting EIF5A. Anti-EIF5A Antibody (4P149) can be used in ELISA, WB, IHC, IF, FCM.
Anti-EIF5A Antibody (4P149)
Anti-EIF5A Antibody (4P149)
😃Good
Catalog No. TMAH-00383Alias eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A
Anti-EIF5A Antibody (4P149) is an antibody targeting EIF5A. Anti-EIF5A Antibody (4P149) can be used in ELISA, WB, IHC, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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| Description | Anti-EIF5A Antibody (4P149) is an antibody targeting EIF5A. Anti-EIF5A Antibody (4P149) can be used in ELISA, WB, IHC, IF, FCM. |
| Alias | eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A |
| Ig Type | Rabbit IgG |
| Clone | 4P149 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate -All lanes: EIF5A antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 17, 21 kDa -Observed band size: 18 kDa 2. IHC image of TMAH-00383 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of Hela Cells with TMAH-00383 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 4. Overlay histogram showing Jurkat cells stained with TMAH-00383 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, IHC, IF, FCM |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; FCM:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human eIF5A |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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