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Anti-Cytokeratin 8 Polyclonal Antibody
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Catalog No. TMAB-00534
Alias KO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2
Anti-Cytokeratin 8 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P.
Anti-Cytokeratin 8 Polyclonal Antibody
Anti-Cytokeratin 8 Polyclonal Antibody
😃Good
Catalog No. TMAB-00534Alias KO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2
Anti-Cytokeratin 8 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Cytokeratin 8 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P. |
| Synonyms | KO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2 |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 8) Polyclonal Antibody, Unconjugated (TMAB-00534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.     |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]. |
| Immunogen | KLH conjugated synthetic peptide: human CK8 |
| Antigen Species | Human |
| Gene Name | KRT8 |
| Gene ID | |
| Protein Name | Keratin, type II cytoskeletal 8 |
| Uniprot ID | |
| Biology Area | Cytokeratins |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Molecular Weight | Theoretical: 53 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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