Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IP

Reactivity: Human

Rabbit IgG

1Q624

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate
-All lanes: SUZ12 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 84 kDa
-Observed band size: 90 kDa
2. IHC image of TMAH-01151 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunoprecipitating SUZ12 in K562 whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-01151 in K562 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-01151(2µg)+ K562 whole cell lysate(500µg)
-Lane 3: K562 whole cell lysate (10µg)

ELISA, WB, IHC, IP

WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human SUZ12

Human

23512

Epigenetics and Nuclear Signaling