IgG1 hFc Protein, Human, Recombinant, Biotinylated

Catalog No. TMPJ-00750 Copy Product Info

IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

IgG1 hFc Protein, Human, Recombinant, Biotinylated

Catalog No. TMPJ-00750

Copy Product Info

IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

Pack Size	Price	USA Stock	Global Stock
5 μg	$129	2-4 weeks	2-4 weeks
10 μg	$213	2-4 weeks	2-4 weeks
20 μg	$355	2-4 weeks	2-4 weeks
50 μg	$625	2-4 weeks	2-4 weeks
100 μg	$963	2-4 weeks	2-4 weeks

Add to Cart

Add to Quotation

For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Resource Download

User Guide of Recombinant Proteins

Product Information

Biological Activity	1. Loaded Biotinylated Human IgG1 Fc-His-Avi on SA Biosensor, can bind Protein G-Fc with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Biotinylated Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Recombinant Protein G. The ED50 of Recombinant Protein G is 49.41 ng/ml. (Regularly tested)
Description	IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.
Species	Human
Expression System	HEK293 Cells
Tag	C-Avi
Accession Number	P01857
Synonyms	IGHG1,g γ-1 chain C region,g gamma-1 chain C region
Amino Acid	Asp104-Lys330(Asp239Glu,Leu241Glu)
Construction	Asp104-Lys330(Asp239Glu,Leu241Glu)
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	33 KDa (reducing condition)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.