IgG1 hFc Protein, Human, Recombinant, Biotinylated

1. Loaded Biotinylated Human IgG1 Fc-His-Avi on SA Biosensor, can bind Protein G-Fc with an affinity constant of <1 pM as determined in BLI assay.  (Regularly tested) 2. Immobilized Biotinylated Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Recombinant Protein G. The ED50 of Recombinant Protein G is 49.41 ng/ml. (Regularly tested)

IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.

Human

HEK293 Cells

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.