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DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi)

(Synonyms: SK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1) Copy Product Info

Synonyms: SK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1

Catalog No. TMPK-00155 Copy Product Info
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$587-10 days7-10 days
10 μg$927-10 days7-10 days
20 μg$1487-10 days7-10 days
50 μg$2897-10 days7-10 days
100 μg$4877-10 days7-10 days
200 μg$8837-10 days7-10 days
500 μg$1,9507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA.
Description
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.
Species
Human
Expression System
HEK293 Cells
TagC-hFc-Avi
Accession NumberO94907
ConstructionMet178-His266
Protein Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSK, hDkk-1, hDkk1, Dkk-1, Dkk1, Dickkopf-related 1, Dickkopf-1
Research Background
Chemical Properties
Molecular Weight38.69 kDa (predicted). Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi) chemical structure | DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi) molecular weight