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Anti-PCNA Antibody (6Q348)
😃Good
Catalog No. TMAH-00858
Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (6Q348) is an antibody targeting PCNA. Anti-PCNA Antibody (6Q348) can be used in ELISA,FCM.
Anti-PCNA Antibody (6Q348)
Anti-PCNA Antibody (6Q348)
😃Good
Catalog No. TMAH-00858Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (6Q348) is an antibody targeting PCNA. Anti-PCNA Antibody (6Q348) can be used in ELISA,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $348 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PCNA Antibody (6Q348) is an antibody targeting PCNA. Anti-PCNA Antibody (6Q348) can be used in ELISA,FCM. |
| Synonyms | proliferating cell nuclear antigen, ATLD2 |
| Ig Type | Mouse IgG2a |
| Clone | 6Q348 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing JK cells stained with TMAH-00858 (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
| Application | |
| Recommended Dose | FCM:1:20-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PCNA Protein |
| Antigen Species | Human |
| Gene Name | PCNA |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Isotype/Loading Controls, Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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