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TNF alpha Protein, Mouse, Recombinant, Biotinylated
(Synonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF) Copy Product InfoSynonyms: tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF
Catalog No. TMPY-05187 Copy Product Info
TNF alpha Protein, Mouse, Recombinant, Biotinylated is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P06804.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $137 | - | In Stock | |
| 10 µg | $230 | - | In Stock | |
| 20 µg | $383 | - | In Stock | |
| 50 µg | $629 | 7-10 days | 7-10 days | |
| 100 µg | $1,050 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95 % as determined by SDS-PAGE.
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Immobilized Recombinant Mouse TNFR1/CD120a/TNFRSF1A Protein (Fc Tag) (Cat#TMPY-02535) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse TNF-alpha/TNFA Protein, Biotinylated (Cat#TMPY-05187), the EC50 is 3-15 ng/mL (QC tested). 2.Immobilized Anti-TNFa (Adalimumab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse TNF-alpha/TNFA Protein, Biotinylated (Cat#TMPY-05187), the EC50 is 3-15 ng/mL (Routinely tested). |
| Description | TNF alpha Protein, Mouse, Recombinant, Biotinylated is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P06804. |
| Species | Mouse |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | P06804 |
| Construction | A DNA sequence encoding the mouse TNF (NP_038721.1) (Leu80-Leu235) was expressed, with an initial Met at the N-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Met |
| Protein Purity | > 95 % as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing Sterile PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | tumor necrosis factor, TNF-α, TNFα, TNFSF2, Tnfsf1a, TNF-alpha, TNFalpha, TNF-a, Tnfa, DIF |
| Research Background | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Molecular Weight | 17.4 kDa (predicted) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: TNF alpha Protein, Mouse, Recombinant, Biotinylated chemical structure | TNF alpha Protein, Mouse, Recombinant, Biotinylated molecular weight
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