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Synonyms: IgG

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 µg | $49 | 7-10 days | 7-10 days | |
| 20 µg | $79 | 7-10 days | 7-10 days | |
| 50 µg | $155 | 7-10 days | 7-10 days | |
| 100 µg | $296 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Rabbit IgG, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5 ng/ml determined by ELISA. |
| Description | IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870. |
| Species | Rabbit |
| Expression System | HEK293 Cells |
| Tag | No Tag |
| Accession Number | P01870 |
| Construction | Ser101-Lys323(T185A, N284S) |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Supplied as 0.22 μm filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4). |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | IgG |
| Research Background | Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. |
| Molecular Weight | 25.13 kDa (Predicted); 30-38 kDa (Due to glycosylation) |
| Shipping | Proteins are shipped with blue ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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