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IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $256 | 7-10 days |
Biological Activity | Immobilized Rabbit IgG, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5 ng/ml determined by ELISA. |
Description | IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870. |
Species | Rabbit |
Expression System | HEK293 Cells |
Tag | No Tag |
Accession Number | P01870 |
Synonyms | IgG |
Construction | Ser101-Lys323(T185A, N284S) |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 25.13 kDa (Predicted); 30-38 kDa (Due to glycosylation) |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Supplied as 0.22 μm filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4). |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. |
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