Immobilized Rabbit IgG, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5 ng/ml determined by ELISA.

IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870.

Rabbit

HEK293 Cells

IgG

Ser101-Lys323(T185A, N284S)

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus.