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IgG Protein, Rabbit, Recombinant (T185A, N284S)

Catalog No. TMPY-02697

IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870.

IgG Protein, Rabbit, Recombinant (T185A, N284S)

IgG Protein, Rabbit, Recombinant (T185A, N284S)

Catalog No. TMPY-02697
IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870.
Pack SizePriceAvailabilityQuantity
100 μg$2567-10 days
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Product Information

Biological Activity
Immobilized Rabbit IgG, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5 ng/ml determined by ELISA.
Description
IgG Protein, Rabbit, Recombinant (T185A, N284S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.13 kDa and the accession number is P01870.
Species
Rabbit
Expression System
HEK293 Cells
TagNo Tag
Accession NumberP01870
Synonyms
IgG
Construction
Ser101-Lys323(T185A, N284S)
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight25.13 kDa (Predicted); 30-38 kDa (Due to glycosylation)
EndotoxinLess than 1EU per μg by the LAL method.
FormulationSupplied as 0.22 μm filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4).
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.