Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC, IF, IP

Reactivity: Human

Rabbit IgG

6G412

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, Brain tissue
-All lanes: KLF4 antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 55, 52, 46, 13, 7 kDa
-Observed band size: 55 kDa
2. IHC image of TMAH-00670 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00670 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
4. Immunofluorescence staining of Hela Cells with TMAH-00670 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
5. Immunoprecipitating KLF4 in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00670 in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00670(2µg)+ Hela whole cell lysate(500µg)
-Lane 3: Hela whole cell lysate (10µg)

ELISA, WB, IHC, IF, IP

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human KLF4

Human

9314

Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Developmental biology, Stem cells