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Anti-FDFT1 Antibody (5J337) is an antibody targeting FDFT1. Anti-FDFT1 Antibody (5J337) can be used in ELISA,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $208 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
| Description | Anti-FDFT1 Antibody (5J337) is an antibody targeting FDFT1. Anti-FDFT1 Antibody (5J337) can be used in ELISA,IHC,WB. |
| Ig Type | Rabbit IgG |
| Clone | 5J337 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate -All lanes: FDFT1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 49, 41, 39, 36, 44 kDa -Observed band size: 50 kDa 2. IHC image of TMAH-00425 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00425 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Tissue Specificity | Widely expressed. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human FDFT1 |
| Antigen Species | Human |
| Gene Name | FDFT1 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Neuroscience, Cancer, Cardiovascular, Metabolism |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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