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Anti-FDFT1 Antibody (5J337)

Catalog No. TMAH-00425 Copy Product Info
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Anti-FDFT1 Antibody (5J337) is an antibody targeting FDFT1. Anti-FDFT1 Antibody (5J337) can be used in ELISA,IHC,WB.

Anti-FDFT1 Antibody (5J337)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00425

Anti-FDFT1 Antibody (5J337) is an antibody targeting FDFT1. Anti-FDFT1 Antibody (5J337) can be used in ELISA,IHC,WB.

Anti-FDFT1 Antibody
(5J337)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2087-10 days7-10 days
100 μL$3497-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-FDFT1 Antibody (5J337) is an antibody targeting FDFT1. Anti-FDFT1 Antibody (5J337) can be used in ELISA,IHC,WB.
Ig Type
Rabbit IgG
Clone
5J337
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate
-All lanes: FDFT1 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 49, 41, 39, 36, 44 kDa
-Observed band size: 50 kDa
2. IHC image of TMAH-00425 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00425 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue SpecificityWidely expressed.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCatalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human FDFT1
Antigen Species
Human
Gene Name
FDFT1
Gene ID
Uniprot ID
Biology Area
Neuroscience, Cancer, Cardiovascular, Metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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