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Alkaline Phosphatase/ALPP Protein, Human, Recombinant (His), Biotinylated
(Synonyms: TMPY-00570, PLAP-1, PLAP1, PLAP 1, PLAP, placental type, Placental alkaline phosphatase 1, PALP, IAP, ALPP and PLAP, ALPI, ALP, alkaline phosphatase, placental, Alkaline phosphatase Regan isozyme, Alkaline phosphatase) Copy Product InfoSynonyms: TMPY-00570, PLAP-1, PLAP1, PLAP 1, PLAP, placental type, Placental alkaline phosphatase 1, PALP, IAP, ALPP and PLAP, ALPI, ALP, alkaline phosphatase, placental, Alkaline phosphatase Regan isozyme, Alkaline phosphatase
Catalog No. TMPK-01575 Copy Product Info
Alkaline Phosphatase/ALPP Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 Cells. The accession number is P05187.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $108 | 7-10 days | 7-10 days | |
| 10 µg | $169 | 7-10 days | 7-10 days | |
| 20 µg | $267 | 7-10 days | 7-10 days | |
| 50 µg | $488 | 7-10 days | 7-10 days | |
| 100 µg | $776 | 7-10 days | 7-10 days | |
| 200 µg | $1,230 | 7-10 days | 7-10 days | |
| 500 µg | $2,290 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). |
| Description | Alkaline Phosphatase/ALPP Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 Cells. The accession number is P05187. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P05187 |
| Construction | Ile23-Asp506 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | TMPY-00570, PLAP-1, PLAP1, PLAP 1, PLAP, placental type, Placental alkaline phosphatase 1, PALP, IAP, ALPP and PLAP, ALPI, ALP, alkaline phosphatase, placental, Alkaline phosphatase Regan isozyme, Alkaline phosphatase |
| Research Background | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
| Molecular Weight | 53.8 kDa (Predicted); 55-70 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Alkaline Phosphatase/ALPP Protein, Human, Recombinant (His), Biotinylated chemical structure | Alkaline Phosphatase/ALPP Protein, Human, Recombinant (His), Biotinylated molecular weight
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