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Anti-CD47 Antibody (1F304)

😃Good
Catalog No. TMAH-00206
Alias OA3, MER6, IAP, CD47 molecule

Anti-CD47 Antibody (1F304) is an antibody targeting CD47. Anti-CD47 Antibody (1F304) can be used in ELISA, FC.

Anti-CD47 Antibody (1F304)

Anti-CD47 Antibody (1F304)

😃Good
Catalog No. TMAH-00206Alias OA3, MER6, IAP, CD47 molecule
Anti-CD47 Antibody (1F304) is an antibody targeting CD47. Anti-CD47 Antibody (1F304) can be used in ELISA, FC.
Pack SizePriceAvailabilityQuantity
50 μL196 € 7-10 days
100 μL329 € 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD47 Antibody (1F304) is an antibody targeting CD47. Anti-CD47 Antibody (1F304) can be used in ELISA, FC.
AliasOA3, MER6, IAP, CD47 molecule
Ig Type
hIgG1
Clone
1F304
Reactivity
Human
Verified Activity
1. The Binding Activity of Human CD47 with Anti-CD47 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Anti-CD47 recombinant antibody. The EC50 is 1.343-1.561 ng/mL.
2. Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-CD47 antibody (TMAH-00206) (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
3. Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (2µg/1*10^6 cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Application
ELISA, FC
Recommended Dose
FC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CD47 Protein
Antigen Species
Human
Gene ID
961
Uniprot ID
Biology Area
Cancer, Immunology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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