B7-H5/Gi24/VISTA Protein, Human, Recombinant (His)

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19 kDa and the accession number is Q9H7M9.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 371.00
500 μg	5 days	$ 1,490.00
1 mg	5 days	$ 2,480.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 371.00

500 μg

5 days

$ 1,490.00

1 mg

5 days

$ 2,480.00

Description

Species	Human
Expression System	HEK293
Tag	C-His
Accession Number	Q9H7M9
Synonyms	VSIR, B7H5, 4632428N05Rik, SISP1, PP2135, Dies1, PD-1H, VISTA, C10orf54, Gi24, PD1H, B7-H5
Construction	Recombinant Human B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe33-Ala194.[Accession \|Q9H7M9]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 32-70 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.