VISTA Protein, Human, Recombinant (His)

Catalog No. TMPK-01186

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19 kDa and the accession number is Q9H7M9.

VISTA Protein, Human, Recombinant (His)

Catalog No. TMPK-01186

Pack Size	Price	Availability
5 μg	$46	7-10 days
10 μg	$72	7-10 days
20 μg	$115	7-10 days
50 μg	$222	7-10 days
100 μg	$371	7-10 days
200 μg	$673	7-10 days
500 μg	$1,490	7-10 days

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Product Information

Biological Activity	Immobilized Human B7-H5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
Description	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19 kDa and the accession number is Q9H7M9.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9H7M9
Synonyms	VSIR,VISTA,SISP1,PP2135,PD-1H,PD1H,Gi24,Dies1,C10orf54,B7-H5,B7H5,4632428N05Rik
Construction	Phe33-Ala194
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	19 kDa (predicted). Due to glycosylation, the protein migrates to 32-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.