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Anti-NPHS2 Polyclonal Antibody
Catalog No. TMAB-09589 Copy Product Info
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Anti-NPHS2 Polyclonal Antibody is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Polyclonal Antibody can be used in WB,ELISA.
Anti-NPHS2 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09589
Anti-NPHS2 Polyclonal Antibody is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Polyclonal Antibody can be used in WB,ELISA.
Anti-NPHS2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NPHS2 Polyclonal Antibody is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Polyclonal Antibody can be used in WB,ELISA. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Recombinant human Podocin, N-his Primary: Anti-NPHS2 (TMAB-09589) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 30 kDa 2. Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 374.9 ng/mL. 3. Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 155.5 ng/mL. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Peripheral membrane protein. |
| Tissue Specificity | Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. |
| Immunogen | Recombinant Protein: human Podocin protein |
| Antigen Species | Human |
| Gene Name | NPHS2 |
| Gene ID | |
| Protein Name | Podocin |
| Uniprot ID |
| Molecular Weight | Theoretical: 42 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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