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Anti-CD81 Polyclonal Antibody

😃Good
Catalog No. TMAB-00395

Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA.

Anti-CD81 Polyclonal Antibody

Anti-CD81 Polyclonal Antibody

😃Good
Catalog No. TMAB-00395
Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Sample:
Lane 1: Recombinant human CD81 protein, hFc (HEK293)
Primary: Anti-CD81 (TMAB-00395) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 36 kDa
2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD81 antibody (TMAB-00395)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,FCM,ELISA
Recommended Dose
WB: 1:500-2000; FCM: 2ug/Test; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TAPA1/CD81
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
CD,Host Virus Interaction,Receptor
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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