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Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-CD81 Polyclonal Antibody is a Rabbit antibody targeting CD81. Anti-CD81 Polyclonal Antibody can be used in WB,FCM,ELISA. |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat) |
Verified Activity | 1. Sample: Lane 1: Recombinant human CD81 protein, hFc (HEK293) Primary: Anti-CD81 (TMAB-00395) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 36 kDa 2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD81 antibody (TMAB-00395) Dilution: 2 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,FCM,ELISA |
Recommended Dose | WB: 1:500-2000; FCM: 2ug/Test; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: human TAPA1/CD81 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | CD,Host Virus Interaction,Receptor |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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