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Anti-SULT2B1 Antibody (6F770)

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Catalog No. TMAY-01857
Alias SULT2B1, sulfotransferase family 2B member 1, HSST2

Anti-SULT2B1 Antibody (6F770) is a Rabbit antibody targeting SULT2B1. Anti-SULT2B1 Antibody (6F770) can be used in WB,ELISA,IP.

Anti-SULT2B1 Antibody (6F770)

Anti-SULT2B1 Antibody (6F770)

😃Good
Catalog No. TMAY-01857Alias SULT2B1, sulfotransferase family 2B member 1, HSST2
Anti-SULT2B1 Antibody (6F770) is a Rabbit antibody targeting SULT2B1. Anti-SULT2B1 Antibody (6F770) can be used in WB,ELISA,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SULT2B1 Antibody (6F770) is a Rabbit antibody targeting SULT2B1. Anti-SULT2B1 Antibody (6F770) can be used in WB,ELISA,IP.
AliasSULT2B1, sulfotransferase family 2B member 1, HSST2
Ig Type
Monoclonal Rabbit IgG
Clone
6F770
Reactivity
Human
Verified Activity
1. SULT2B1 was immunoprecipitated using:

-Lane A:0.5 mg MCF-7 Whole Cell Lysate

-0.5 µL anti-SULT2B1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-SULT2B1 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 41 kDa.

-Observed band size: 41 kDa.

2. Anti-SULT2B1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: MCF7 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:41 kDa.

-Observed band size:41 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human SULT2B1 (rh SULT2B1; TMPY-01616; NP_004596.2; Asp 2-Ser 365) .
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSulfotransferase family cytosolic 2B member 1, also known as Sulfotransferase 2B1, ST2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, SULT2B1 and HSST2, is a cytoplasm protein that belongs to the sulfotransferase 1 family. The human hydroxysteroid sulfotransferase (SULT) family is comprised of two subfamilies, SULT2A1 and SULT2B1. SULT2B1 is expressed highly in placenta, prostate and trachea. A lesser expression of SULT1B1 was observed in the small intestine and lung. SULT2B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol. The two SULT2B1 isoforms, SULT2B1a and SULT2B1b, are encoded by a single gene as a result of alternative transcription initiation and alternative splicing. SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human SULT2B1 Protein (TMPY-01616)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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