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Anti-COX5B Antibody (3I109)
😃Good
Catalog No. TMAY-02788
Alias cytochrome c oxidase subunit Vb, COXVB
Anti-COX5B Antibody (3I109) is a Rabbit antibody targeting COX5B. Anti-COX5B Antibody (3I109) can be used in WB,ELISA,IP.
Anti-COX5B Antibody (3I109)
Anti-COX5B Antibody (3I109)
😃Good
Catalog No. TMAY-02788Alias cytochrome c oxidase subunit Vb, COXVB
Anti-COX5B Antibody (3I109) is a Rabbit antibody targeting COX5B. Anti-COX5B Antibody (3I109) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Chemical Properties
| Description | Anti-COX5B Antibody (3I109) is a Rabbit antibody targeting COX5B. Anti-COX5B Antibody (3I109) can be used in WB,ELISA,IP. |
| Alias | cytochrome c oxidase subunit Vb, COXVB |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3I109 |
| Reactivity | Human |
| Verified Activity | 1. Anti-COX5B rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:14 kDa. -Observed band size:15 kDa. 2. COX5B was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-COX5B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-COX5B rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 14 kDa. -Observed band size: 14 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human COX5B (rh COX5B; TMPY-03511; P10606; Ala32-His129). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human COX5B Protein (TMPY-03511) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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