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Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
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Catalog No. TMAY-03070
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) is a Rabbit antibody targeting 4-1BB Ligand/TNFSF9. Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) can be used in ELISA,FCM,ICC/IF.
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
😃Good
Catalog No. TMAY-03070
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) is a Rabbit antibody targeting 4-1BB Ligand/TNFSF9. Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $270 | 7-10 days |
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Chemical Properties
| Description | Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) is a Rabbit antibody targeting 4-1BB Ligand/TNFSF9. Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8F465 |
| Reactivity | Mouse |
| Verified Activity | 1. Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFSF9 / 4-1BBL (rM TNFSF9; TMPY-00959; Arg 104-Glu 309; NP_033430.1). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | 4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Mouse TNFSF9 protein (TMPY-00959) |
| Antigen Species | Mouse |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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