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Anti-Osteocalcin Polyclonal Antibody 2
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Catalog No. TMAB-01296
Anti-Osteocalcin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Osteocalcin Polyclonal Antibody 2
Anti-Osteocalcin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01296
Anti-Osteocalcin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,ELISA Reactivity: Human,Mouse (predicted:Rat) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human MG63 cell lysates Lane 3: Human A549 cell lysates Lane 4: Human U-2 OS cell lysates Primary: Anti-Osteocalcin (TMAB-01296) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus stainin 3. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining. 4. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. |
| Immunogen | KLH conjugated synthetic peptide: human Osteocalcin |
| Antigen Species | Human |
| Gene ID | 632 |
| Uniprot ID | |
| Biology Area | Bone,Osteogenesis |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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