Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IF, FC

Reactivity: Human

IgG2a

8P93

Human

1. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate
-All lanes: CD146 antibody at 1:1250
-Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution
-Predicted band size: 72, 58 kDa
-Observed band size: 120 kDa
2. Western Blot
-Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
-All lanes: CD146 antibody at 1:500
-Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution
-Predicted band size: 72, 58 kDa
-Observed band size: 120 kDa
3. Immunofluorescence staining of A375 cells with TMAH-00169 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
4. Immunofluorescence staining of Hela cells with TMAH-00169 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
5. Immunofluorescence staining of MCF-7 cells with TMAH-00169 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescence staining of THP-1 cells with TMAH-00169 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Overlay histogram showing MCF-7 cells stained with TMAH-00169 (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FC

Monoclonal

Unconjugated

Recombinant Protein: Human Cell surface glycoProtein MUC18 Protein (50-646AA)

Human

4162

Immunology