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Anti-MGMT Antibody (9E151) (Synonyms: O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cy...)
Catalog No. TMAH-00755 Copy Product Info
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Anti-MGMT Antibody (9E151) is an antibody targeting MGMT. Anti-MGMT Antibody (9E151) can be used in ELISA,IHC,WB.
Anti-MGMT Antibody (9E151)
Copy Product Info🥰Excellent
Catalog No. TMAH-00755
Synonyms O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase
Anti-MGMT Antibody (9E151) is an antibody targeting MGMT. Anti-MGMT Antibody (9E151) can be used in ELISA,IHC,WB.
Anti-MGMT Antibody
(9E151)
(9E151)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-MGMT Antibody (9E151) is an antibody targeting MGMT. Anti-MGMT Antibody (9E151) can be used in ELISA,IHC,WB. |
| Synonyms | O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase |
| Ig Type | Rabbit IgG |
| Clone | 9E151 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate -All lanes: MGMT antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 22 kDa -Observed band size: 22 kDa 2. IHC image of TMAH-00755 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human MGMT |
| Antigen Species | Human |
| Gene Name | MGMT |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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