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IL-12 Protein, Human, Recombinant (His), Biotinylated
(Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF) Copy Product InfoSynonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF
Catalog No. TMPY-05458 Copy Product Info
IL-12 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459.2&NP_002178.2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $137 | 7-10 days | 7-10 days | |
| 10 μg | $230 | 7-10 days | 7-10 days | |
| 20 μg | $383 | 7-10 days | 7-10 days | |
| 50 μg | $629 | 7-10 days | 7-10 days | |
| 100 μg | $1,050 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Immobilized Recombinant Human IL-12 R beta 2/IL12RB2 Protein (His Tag) (Cat#TMPY-07002) at 5 μg/mL (100 μL/well) can bind Recombinant Human IL-12 (IL12A & IL12B Heterodimer) Protein, Biotinylated (Cat#TMPY-05458), the EC50 is 30-100 ng/mL (Routinely tested). 2.Immobilized Recombinant Human IL-12 (IL12A & IL12B Heterodimer) Protein, Biotinylated (Cat#TMPY-05458) at 1 μg/mL (100 μL/well) on Streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Anti-IL-12 & IL-23 (P40)(Ustekinumab Biosimilar), the EC50 is 1.1-3.5 ng/mL (Routinely tested). |
| Description | IL-12 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459.2&NP_002178.2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P29459.2&NP_002178.2 |
| Construction | A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Arg 23 & Ile 23 |
| Protein Purity | > 95 % as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF |
| Molecular Weight | 60.13 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: IL-12 Protein, Human, Recombinant (His), Biotinylated chemical structure | IL-12 Protein, Human, Recombinant (His), Biotinylated molecular weight
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