Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi)

Name: Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi)
Brand: TargetMol
SKU: TMPK-01748
Rating: 4.5 (1 reviews)

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Catalog No. TMPK-01748 Copy Product Info

Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 Cells. The accession number is P13612-1(ITGA4)&P05556-1(ITGB1).

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human ITGA4&ITGB1, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 7.0 ng/ml determined by ELISA (QC Test).
Description	Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 Cells. The accession number is P13612-1(ITGA4)&P05556-1(ITGB1).
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P13612-1 (ITGA4) & P05556-1 (ITGB1)
Construction	Tyr34-Thr977 (ITGA4) acidic tail and Gln21-Asp728 (ITGB1) basic tail
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin	< 1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background

Chemical Properties

Molecular Weight

112.5 kDa (ITGA4) and 83.2 kDa (ITGB1) (Predicted); 70-90 kDa and 120-160 kDa (Reducing condition, due to glycosylation)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi) chemical structure | Integrin alpha 4 beta 1 Protein, Human, Recombinant (His & Avi) molecular weight