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Anti-Phospho-MDM2 (Ser166) Antibody (2E620)
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Catalog No. TMAH-00927
Alias RING-type E3 ubiquitin transferase Mdm2, p-MDM2 (Ser166), p-MDM2 (S166), Phospho-MDM2 (S166), p53-binding protein Mdm2, Oncoprotein Mdm2, MDM2 (p-Ser166), MDM2 (p-S166), MDM 2, Hdm2, EC 2.3.2.27, E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein
Anti-Phospho-MDM2 (Ser166) Antibody (2E620) is an antibody targeting Phospho-MDM2 (Ser166). Anti-Phospho-MDM2 (Ser166) Antibody (2E620) can be used in ELISA, IHC, IF, FCM.
Anti-Phospho-MDM2 (Ser166) Antibody (2E620)
Anti-Phospho-MDM2 (Ser166) Antibody (2E620)
😃Good
Catalog No. TMAH-00927Alias RING-type E3 ubiquitin transferase Mdm2, p-MDM2 (Ser166), p-MDM2 (S166), Phospho-MDM2 (S166), p53-binding protein Mdm2, Oncoprotein Mdm2, MDM2 (p-Ser166), MDM2 (p-S166), MDM 2, Hdm2, EC 2.3.2.27, E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein
Anti-Phospho-MDM2 (Ser166) Antibody (2E620) is an antibody targeting Phospho-MDM2 (Ser166). Anti-Phospho-MDM2 (Ser166) Antibody (2E620) can be used in ELISA, IHC, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC, IF, FCM Reactivity: Human |
| Alias | RING-type E3 ubiquitin transferase Mdm2, p-MDM2 (Ser166), p-MDM2 (S166), Phospho-MDM2 (S166), p53-binding protein Mdm2, Oncoprotein Mdm2, MDM2 (p-Ser166), MDM2 (p-S166), MDM 2, Hdm2, EC 2.3.2.27, E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein |
| Ig Type | Rabbit IgG |
| Clone | 2E620 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00927 diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB. 2. IHC image of TMAH-00927 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB. 3. Immunofluorescence staining of HepG2 with TMAH-00927 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 4. Overlay Peak curve showing Hela cells stained with TMAH-00927 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IHC, IF, FCM |
| Recommended Dose | IHC:1:50-1:200; IF:1:50-1:200; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Nucleus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human MDM2 |
| Antigen Species | Human |
| Gene ID | 4193 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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