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TMB
🥰Excellent
Catalog No. T19071Cas No. 54827-17-7
Alias Sure Blue TMB, BM blue
TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA.
TMB
🥰Excellent
Purity: 99.96%
Catalog No. T19071Alias Sure Blue TMB, BM blueCas No. 54827-17-7
TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 1 g | $33 | In Stock | |
| 1 mL x 10 mM (in DMSO) | $30 | In Stock |
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Purity:99.96%
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Product Introduction
Bioactivity
Chemical Properties
| Description | TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA. |
| Cell Research | Procedure 1. Prepare bacterial cell culture: E. coli cells were grown in LB medium at 37°C to a suitable state. 2. Collect cells by centrifugation and wash 3 times with PBS. 3. Incubate cells with TMB (200 μM) for 10 minutes. 4. Wash 3 times with PBS to remove excess TMB. 5. Collect optical signals. |
| Synonyms | Sure Blue TMB, BM blue |
| Molecular Weight | 240.34 |
| Formula | C16H20N2 |
| Cas No. | 54827-17-7 |
| Smiles | Cc1cc(cc(C)c1N)-c1cc(C)c(N)c(C)c1 |
| Relative Density. | 1 g/cm3 |
| Color | White |
| Appearance | Solid |
Storage & Solubility Information
| Storage | keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 262 mg/mL (1090.12 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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