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Synonyms: leIF A, IFN-alphaA, IFN-alpha-2, IFNA2C, IFNA2B, IFNA2A, IFNA


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $167 | 7-10 days | 7-10 days | |
| 10 μg | $277 | 7-10 days | 7-10 days | |
| 20 μg | $462 | 7-10 days | 7-10 days | |
| 50 μg | $743 | 7-10 days | 7-10 days | |
| 100 μg | $1,220 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated (Cat#TMPY-07119) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind IFNAR2 Protein, Human, Recombinant (hFc) (Cat#TMPY-01640), the EC50 is 12-40 ng/mL. |
| Description | IFN-alpha 2b/IFNA2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01563-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P01563-1 |
| Construction | A DNA sequence encoding the Human Interferon alpha 2 / IFNA2 (P01563-1) (Met1-Glu188) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | leIF A, IFN-alphaA, IFN-alpha-2, IFNA2C, IFNA2B, IFNA2A, IFNA |
| Research Background |
| Molecular Weight | 22.53 kDa (predicted); 25.1 kDa and 23.5 kDa (reducing contition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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