HVEM Protein, Mouse, Recombinant (His & hFc)

Name: HVEM Protein, Mouse, Recombinant (His & hFc)
Brand: TargetMol
SKU: TMPY-01425
Rating: 4.5 (1 reviews)

(Synonyms: tumor necrosis factor receptor superfamily, member 14, Tnfrs14, Hvem, HveA, Atar) Copy Product Info

Synonyms: tumor necrosis factor receptor superfamily, member 14, Tnfrs14, Hvem, HveA, Atar

Catalog No. TMPY-01425 Copy Product Info

HVEM Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 46.4 kDa and the accession number is Q80WM9.

Pack Size	Price	EUR Stock	Global Stock
5 µg	52 €	7-10 days	7-10 days
10 µg	83 €	7-10 days	7-10 days
20 µg	136 €	7-10 days	7-10 days
50 µg	268 €	7-10 days	7-10 days
100 µg	519 €	7-10 days	7-10 days
200 µg	772 €	7-10 days	7-10 days
500 µg	1.665 €	7-10 days	7-10 days
1 mg	3.411 €	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized Mouse BTLA hFc at 2 μg/ml (100 μl/well) can bind Mouse HVEM Fch , the EC50 of Mouse HVEM Fch is 2-10 ng/mL.
Description	HVEM Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 46.4 kDa and the accession number is Q80WM9.
Species	Mouse
Expression System	HEK293 Cells
Tag	His, hFc
Accession Number	Q80WM9
Construction	A DNA sequence encoding the extracellular domain (Met 1-Gln 206) of mouse HVEM (NP_849262.1) precursor was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus. Predicted N terminal: Pro 40
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	tumor necrosis factor receptor superfamily, member 14, Tnfrs14, Hvem, HveA, Atar
Research Background	Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

46.4 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.