Beta-NGF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-01010U

Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

Beta-NGF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-01010U

Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

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Biological Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml. (QC Test) Immobilized Human Beta-NGF, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25 μg/ml determined by ELISA.
Description	Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.
Species	Human
Expression System	HEK293 Cells
Accession Number	P01138
Synonyms	β-NGF,NGFB,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide),HSAN5,Beta-NGF
Construction	Ser122-Ala241
Protein Purity	> 95% as determined by Bis-Tris PAGE
Molecular Weight	13.49 kDa (Predicted); 14-18 kDa (Due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).