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Beta-NGF Protein, Human, Recombinant (HEK293)

(Synonyms: β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF) Copy Product Info

Synonyms: β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF

Catalog No. TMPY-01010U Copy Product Info
Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.
Beta-NGF Protein, Human, Recombinant (HEK293)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$40-In Stock
10 µg$61-In Stock
20 µg$967-10 days7-10 days
50 µg$1837-10 days7-10 days
100 µg$306-In Stock
200 µg$4987-10 days7-10 days
500 µg$9597-10 days7-10 days
1 mg$1,5807-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml. (QC Test)
Immobilized Human Beta-NGF, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25 μg/ml determined by ELISA.
Description
Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberP01138
ConstructionSer122-Ala241
Protein Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in 20mM NaAc, 150mM Nacl (pH 5.5). The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonymsβ-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF
Research Background
Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).
Chemical Properties
Molecular Weight13.49 kDa (Predicted); 14-18 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: Beta-NGF Protein, Human, Recombinant (HEK293) chemical structure | Beta-NGF Protein, Human, Recombinant (HEK293) molecular weight