Beta-NGF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-01010U Copy Product Info

Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

Beta-NGF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-01010U

Copy Product Info

Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

Pack Size	Price	USA Stock	Global Stock
5 μg	$40	-	In Stock
10 μg	$61	-	In Stock
20 μg	$96	7-10 days	7-10 days
50 μg	$183	7-10 days	7-10 days
100 μg	$306	-	In Stock
200 μg	$498	7-10 days	7-10 days
500 μg	$959	7-10 days	7-10 days
1 mg	$1,580	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml. (QC Test) Immobilized Human Beta-NGF, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25 μg/ml determined by ELISA.
Description	Beta-NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P01138
Synonyms	β-NGF,NGFB,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide),HSAN5,Beta-NGF
Construction	Ser122-Ala241
Protein Purity	> 95% as determined by Bis-Tris PAGE
Molecular Weight	13.49 kDa (Predicted); 14-18 kDa (Due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 20mM NaAc, 150mM Nacl (pH 5.5). The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).