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Anti-B7-H3 Antibody (7G630) (Synonyms: CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3)

Catalog No. TMAY-01732 Copy Product Info
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Anti-B7-H3 Antibody (7G630) is a Mouse antibody targeting B7-H3. Anti-B7-H3 Antibody (7G630) can be used in ELISA,ELISA(Det),FCM,ICC/IF.

Anti-B7-H3 Antibody (7G630)

Copy Product Info
🥰Excellent
Catalog No. TMAY-01732
Synonyms CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3

Anti-B7-H3 Antibody (7G630) is a Mouse antibody targeting B7-H3. Anti-B7-H3 Antibody (7G630) can be used in ELISA,ELISA(Det),FCM,ICC/IF.

Anti-B7-H3 Antibody
(7G630)
Pack SizePriceUSA StockGlobal StockQuantity
20 μL$697-10 days7-10 days
100 μL$1987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-B7-H3 Antibody (7G630) is a Mouse antibody targeting B7-H3. Anti-B7-H3 Antibody (7G630) can be used in ELISA,ELISA(Det),FCM,ICC/IF.
SynonymsCD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
Ig Type
Mouse IgG2b
Clone
7G630
Reactivity
Human
Specificity
Human B7-H3
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Verified Activity
1. Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
2. Flow cytometric analysis of Human B7H3(CD276) expression on PC-3 cells. Cells were stained with purified anti-Human B7H3(CD276), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
Recommended Dose
ELISA: 1:1000-1:2000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; TMPY-02031; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundB7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/HRP/PE
Antibody Types Available
5
Antigen Details
Immunogen
Recombinant Protein: Human B7-H3 / CD276 protein (TMPY-02031)
Antigen Species
Human
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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