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Anti-B7-H3 Antibody (7G630)
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Catalog No. TMAY-01732
Alias CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
Anti-B7-H3 Antibody (7G630) is a Mouse antibody targeting B7-H3. Anti-B7-H3 Antibody (7G630) can be used in ELISA,ELISA(Det),FCM,ICC/IF.
Anti-B7-H3 Antibody (7G630)
Anti-B7-H3 Antibody (7G630)
😃Good
Catalog No. TMAY-01732Alias CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
Anti-B7-H3 Antibody (7G630) is a Mouse antibody targeting B7-H3. Anti-B7-H3 Antibody (7G630) can be used in ELISA,ELISA(Det),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, ELISA(Det), FCM, ICC/IF Reactivity: Human |
| Alias | CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3 |
| Ig Type | Mouse IgG2b |
| Clone | 7G630 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Human B7H3(CD276) expression on PC-3 cells. Cells were stained with purified anti-Human B7H3(CD276), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,ELISA(Det),FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; TMPY-02031; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/HRP/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Human B7-H3 / CD276 protein (TMPY-02031) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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