Anti-SPARC Polyclonal Antibody 2 is a Rabbit antibody targeting SPARC. Anti-SPARC Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (TMAB-13079) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (TMAB-13079) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse B16 cell lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-SPARC (TMAB-13079) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
4. Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/mL.
5. Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/mL.

WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human SPARC protein

Human

SPARC

SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.