Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, FC

Reactivity: Human, Mouse

Mouse IgG2a

9S2

Human, Mouse

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue lysate
-All lanes: ANXA4 antibody at 1:500
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 36 kDa
-Observed band size: 36 kDa
2. Overlay Peak curve showing Hela cells stained with TMAH-00066 (red line) at 1:800. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,017 events was performed.

ELISA, WB, FC

WB:1:1000-1:5000; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human ANXA4 Protein

Human

307

Cardiovascular, Signal transduction