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Anti-NCAM Antibody (6L815) is an antibody targeting NCAM. Anti-NCAM Antibody (6L815) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | neural cell adhesion molecule 1, NCAM, MSK39, CD56 |
Ig Type | Rabbit IgG |
Clone | 6L815 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: SY5Y whole cell lysate -All lanes: NCAM antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 120-220 kDa -Observed band size: 150 kDa 2. IHC image of TMAH-00805 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 4]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human NCAM |
Antigen Species | Human |
Gene ID | 4684 |
Uniprot ID | |
Biology Area | Neuroscience, Signal transduction, Stem cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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