B7-2/CD86 Protein, Rat, Recombinant (His)

Name: B7-2/CD86 Protein, Rat, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02016
Rating: 4.5 (1 reviews)

(Synonyms: CD86 antigen) Copy Product Info

Synonyms: CD86 antigen

Catalog No. TMPY-02016 Copy Product Info

CD86 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is A0A9K3Y6P6.

B7-2/CD86 Protein, Rat, Recombinant (His)

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear range of 6.4-160 ng/ml.
Description	CD86 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is A0A9K3Y6P6.
Species	Rat
Expression System	HEK293 Cells
Tag	C-His
Accession Number	NP_064466.1
Construction	A DNA sequence encoding the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Pro 30
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	CD86 antigen
Research Background	CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

26.8 kDa (predicted); 40-70 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.