Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05580

CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.

CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)

CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05580
CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.
Pack SizePriceAvailabilityQuantity
100 μg$6007-10 days
1 mg$3,9107-10 days
Bulk & Custom
Add to Cart
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View More

Product Information

Biological Activity
Immobilized Human CD73, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6 ng/ml determined by ELISA (QC Test).
Description
CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP21589-1
Synonyms
NTE,NT5,NT,eNT,eN,E5NT,CD73,CALJA,5'-nucleotidase, ecto (CD73)
Construction
Trp27-Lys547
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight84.5 kDa (Predicted); 85-100 kDa (Due to glycosylation)
EndotoxinLess than 1EU per μg by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.

Dose Conversion

You can also refer to dose conversion for different animals. More

Sci Citations

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords